{"entry": {"accession": "MF7000233", "general": {"name": "DR2232 (E46A mutant)", "pdb_id": "5hwu", "exp_method": "X-ray", "resolution": "2.10", "assembly": "Homodimer", "source_organism": "Deinococcus radiodurans", "publication": {"pmid": "27739259", "authors": "Mota CS, Gon\u00e7alves AM, de Sanctis D", "title": "Deinococcus radiodurans DR2231 is a two-metal-ion mechanism hydrolase with exclusive activity on dUTP.", "journal": "FEBS J.", "year": "2016", "issue": "23", "volume": "283", "pages": "4274-4290"}}, "function": {"molecular_function": {"go": [{"accession": "GO:0046872", "name": "metal ion binding"}, {"accession": "GO:0047429", "name": "nucleoside triphosphate diphosphatase activity"}]}}, "macromolecules": {"general": {"nr_of_chains": "2", "nr_of_unique_protein_segments": "1", "class": "Homooligomeric enzymes", "subclass": "Homodimeric enzymes", "note": "All chains according to the most probable oligomerization state stored in PDBe were considered."}, "chain": [{"id": "A", "name": "HAD superfamily Cof-like phosphohydrolase", "source_organism": "Deinococcus radiodurans", "uniprot": {"id": "Q9RS96", "start": "7", "end": "144", "coverage": "93%", "sequence": "MSDLPCPPTNAERLHEFHRAIGAATPERPTPPPPELLRLRQTLLDEESAEVRAEIDHLLARQAAGEALSAGDLAPLAHELADLLYVTYGALDQLGIDADAVFAEVHRANLSKASGPRRADGKQLKPEGWRPADVRGVIERLQHAPADD", "length": "148"}, "regions": {"region": [{"region_type": "secondary structure", "region_name": "helix", "region_start": "3", "region_end": "15"}, {"region_type": "secondary structure", "region_name": "helix", "region_start": "27", "region_end": "58"}, {"region_type": "secondary structure", "region_name": "helix", "region_start": "64", "region_end": "66"}, {"region_type": "secondary structure", "region_name": "helix", "region_start": "67", "region_end": "88"}, {"region_type": "secondary structure", "region_name": "helix", "region_start": "91", "region_end": "105"}, {"region_type": "secondary structure", "region_name": "helix", "region_start": "127", "region_end": "137"}, {"region_type": "pfam", "region_id": "PF01503", "region_name": "Phosphoribosyl-ATP pyrophosphohydrolase", "region_start": "14", "region_end": "109"}]}}, {"id": "B", "name": "HAD superfamily Cof-like phosphohydrolase", "source_organism": "Deinococcus radiodurans", "uniprot": {"id": "Q9RS96", "start": "7", "end": "144", "coverage": "93%", "sequence": "MSDLPCPPTNAERLHEFHRAIGAATPERPTPPPPELLRLRQTLLDEESAEVRAEIDHLLARQAAGEALSAGDLAPLAHELADLLYVTYGALDQLGIDADAVFAEVHRANLSKASGPRRADGKQLKPEGWRPADVRGVIERLQHAPADD", "length": "148"}, "regions": {"region": [{"region_type": "secondary structure", "region_name": "helix", "region_start": "3", "region_end": "15"}, {"region_type": "secondary structure", "region_name": "helix", "region_start": "27", "region_end": "58"}, {"region_type": "secondary structure", "region_name": "helix", "region_start": "64", "region_end": "66"}, {"region_type": "secondary structure", "region_name": "helix", "region_start": "67", "region_end": "88"}, {"region_type": "secondary structure", "region_name": "helix", "region_start": "91", "region_end": "107"}, {"region_type": "secondary structure", "region_name": "helix", "region_start": "127", "region_end": "137"}, {"region_type": "pfam", "region_id": "PF01503", "region_name": "Phosphoribosyl-ATP pyrophosphohydrolase", "region_start": "14", "region_end": "109"}]}}]}, "evidence": {"evidence_level": "Direct evidence", "evidence_coverage": "The full structure participates in mutual synergistic folding.", "sequence_domain": "Phosphoribosyl-ATP pyrophosphohydrolase", "complex_evidence": "Phosphoribosyl-ATP pyrophosphohydrolases show a very unusual interlaced segment-swapped dimer, which implies that this obligatory dimer assembly is important for their function. Size-exclusion chromatography combined with static light scattering confirmed that the dimer is the major oligomeric state in solution (PMID:20944217). Upon dimer formation, DR2231 helices 2 and 3 from one monomer stack antiparallel to helices 2\u2032 and 3\u2032 of the other monomer, respectively. A stable four-helix bundle is formed in the center. The intertwining of the two hairpin structures produces an extensive subunit-subunit interface (PMID:21733847).", "chain_evidence": [{"chain_id": "A", "support": "N/A"}, {"chain_id": "B", "support": "N/A"}]}, "related_structures": {"id": ["MF7000284", "MF7000232", "MF7000233"]}}}